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Registros recuperados: 74 | |
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Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco. |
In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides... |
Tipo: Text |
Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf |
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Bigot, Laetitia; Zatylny-gaudin, Celine; Rodet, Franck; Bernay, Benoit; Boudry, Pierre; Favrel, Pascal. |
Gonadotropin-releasing hormone (GnRH), a key neuropeptide regulating reproduction in vertebrates has now been characterized in a number of non-vertebrate species. Despite the demonstration of its ancestral origin, the structure and the function of this family of peptides remain poorly known in species as distant as lophotrochozoans. In this study, two GnRH-related peptides (Cg-GnRH-a and CgGnRH-G) were characterized by mass spectrometry from extracts of the visceral ganglia of the Pacific oyster Crassostrea gigas. These peptides showed a high degree of sequence identity with GnRHs of other mollusks and annelids and to a lesser extent with those of vertebrates or with AKH and corazonins of insects. Both the mature peptides and the transcript encoding the... |
Tipo: Text |
Palavras-chave: Mollusk; Bivalve; Oyster; Neuroendocrinology; Reproduction; GnRH. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00079/19003/17477.pdf |
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Pernet, Fabrice; Malet, Nathalie; Pastoureaud, Annie; Vaquer, Andre; Quere, Claudie; Dubroca, Laurent. |
Carbon stable isotopes and fatty acids were measured in the suspended particulate organic matter (POM) of the Thau lagoon to study its qualitative temporal changes in relation to environmental factors and to identify the food sources of bivalves over a one-yr-cycle in relation to their growth. Reciprocally, the impact of shellfish farming on POM was also studied. Oysters and mussels were sampled and measured for biometry, stable isotopes and fatty acid composition. Water samples were collected at two sites, both inside and outside of the shellfish farming area, to determine concentrations in POM, chlorophyll a (Chl a) and stable isotopes. Carbon isotopes and fatty acids in bivalves reflected seasonal changes in food sources, which varied consistently with... |
Tipo: Text |
Palavras-chave: Aquaculture; Bivalve; Competition; Feeding ecology; Growth; Trophic markers. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00075/18575/16177.pdf |
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Suquet, Marc; Quere, Claudie; Mingant, Christian; Lebrun, Luc; Ratiskol, Dominique; Miner, Philippe; Cosson, Jacky. |
Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed... |
Tipo: Text |
Palavras-chave: Spermatozoa; CASA; Maturation; Sperm motility; Bivalve; Pecten maximus. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00157/26852/24983.pdf |
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Rossi, Francesca; Colao, Elodie; Jose Martinez, Maria; Klein, Judith C.; Carcaillet, Frederique; Callier, Myriam; De Wit, Rutger; Caro, Audrey. |
Sulphur-oxidising endosymbiont-bearing bivalves often inhabit seagrass meadows, where they can control sulphide levels and variably contribute to carbon cycling, by feeding on endosymbiotic bacteria and/or on particulate organic matter from the water column. The patterns of variability in their feeding mode and their spatial distribution within the seagrass meadows are however poorly studied. Seagrass beds form naturally patchy habitats with seagrass-sand edges that may have variable effects on different organisms. The present study aims at understanding differences in feeding mode and abundance of the endosymbiont-bearing bivalve Loripes lacteus (sensu Poli, 1791) as well as the physiological conditions of its endosymbiotic populations between edge and... |
Tipo: Text |
Palavras-chave: Bivalve; Food web; Mediterranean; Seagrass; Stable isotopes; Symbiosis. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00138/24911/26373.pdf |
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Caill-milly, Nathalie; Bru, Noelle; Barranger, Melanie; Gallon, Laurent; D'Amico, Franck. |
For species' dynamics highly influenced by environmental conditions, sustainable management for conservation and/or exploitation purposes requires the ability to segregate natural processes from anthropogenic impacts. In this context, identification of phenotypic variations in morphological patterns, and clarification of the mechanisms involved, constitute useful tools to identify adequate management measures from Europe-wide to local conditions. Although such approaches are already used effectively in the management of fish stocks, they are more recent for bivalve stocks, which are also good candidates for such management tools. The current study examines morphological patterns in the Manila clam (Venerupis philippinarum) through morphometric studies of 4... |
Tipo: Text |
Palavras-chave: Shell shape; Linear morphometric methods; Contour detection; Environmental effect; Linear multilevel model; Bivalve; Atlantic Ocean; Venerupis philippinarum. |
Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00225/33605/32004.pdf |
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Lassudrie, Malwenn; Soudant, Philippe; Richard, Gaelle; Henry, N.; Medhioub, Walid; Da Silva, P.m.; Donval, Anne; Bunel, M.; Le Goïc, Nelly; Lambert, Christophe; De Montaudoin, X.; Fabioux, Caroline; Hegaret, Helene. |
Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the... |
Tipo: Text |
Palavras-chave: Bivalve; Harmful algal bloom; Oxidative stress; Venerupis philippinarum; Alexandrium ostenfeldii; Perkinsus olseni.. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00189/30022/28507.pdf |
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Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan. |
Pesticides are frequently detected in estuaries among the pollutants found in estuarine and coastal areas and may have major ecological consequences. They could endanger organism growth, reproduction or survival. In the context of high mortality outbreaks affecting Pacific oysters, Crassostrea gigas, in France since 2008, it appears of importance to determine the putative effects of pesticides on oyster susceptibility to infectious agents. Massive mortality outbreaks reported in this species, mainly in spring and summer, may suggest an important role played by the seasonal use of pesticides and freshwater input in estuarine areas where oyster farms are frequently located. To understand the impact of some pesticides detected in French waters, their effects... |
Tipo: Text |
Palavras-chave: Immunity; Hemocytes; Pesticides; Pacific oyster; Crassostrea gigas; Flow cytometry; Bivalve. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00144/25555/23700.pdf |
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Pernet, Fabrice; Tamayo, David; Petton, Bruno. |
Mortalities of oyster seed of Crassostrea gigas associated with ostreid herpes virus OsHV-1 μVar have been observed in many oyster producing countries since 2008. The objective of this study was to further investigate whether low temperature treatments can offer a viable option to mitigate oyster mortalities. An experiment was set-up to further evaluate the effect of low temperature treatments (10 and 13°C vs. 21°C) and their duration (6 d to 83 d) on the survival of oysters previously infected with OsHV-1 μVar by means of exposure to field conditions in areas where mortalities were occurring. Average survival of oysters infected with OsHV-1μVar was 71% after 83 d at low temperatures compared to only 23% in controls maintained at 21°C. During... |
Tipo: Text |
Palavras-chave: Bivalve; Disease; OsHV-1; Oyster; Temperature; Virus. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00259/37047/35555.pdf |
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Noble, Mae; Fenner School of Environment and Society, The Australian National University; mae.noble@anu.edu.au; Duncan, Phil; Gamilaroi Traditional Owner, NSW Aboriginal Land Council; phil.duncan@alc.org.au; Perry, Darren; Murray Lower Darling Rivers Indigenous Nations; ngintait@gmail.com; Prosper, Kerry; Paq'tnekek Mi'kmaq First Nations; kerryp@paqtnkek.ca; Rose, Denis; Gunditj Mirring Traditional Owners Aboriginal Corporation; Denis@gunditjmirring.com; Schnierer, Stephan; School of Environment, Science and Engineering, Southern Cross University; stephan.schnierer@scu.edu.au; Tipa, Gail; Tipa and Associates Ltd.; gttipa@vodafone.co.nz; Williams, Erica; National Institute of Water and Atmospheric Research; Erica.Williams@niwa.co.nz; Woods, Rene; National Cultural Flows Program; Murray Lower Darling River Indigenous Nations; woodsre83@gmail.com; Pittock, Jamie; Fenner School of Environment and Society, The Australian National University; jamie.pittock@anu.edu.au. |
Indigenous peoples of North America, Australia, and New Zealand have a long tradition of harvesting freshwater animals. Over generations of reliance and subsistence harvesting, Indigenous peoples have acquired a profound understanding of these freshwater animals and ecosystems that have become embedded within their cultural identity. We have identified trans-Pacific parallels in the cultural significance of several freshwater animal groups, such as eels, other finfish, bivalves, and crayfish, to Indigenous peoples and their understanding and respect for the freshwater ecosystems on which their community survival depends. In recognizing such cultural connections, we found that non-Indigenous peoples can appreciate the deep significance of freshwater animals... |
Tipo: Peer-Reviewed Synthesis |
Palavras-chave: Adaptive freshwater management; Aquatic resources; Bivalve; Comanagement; Crayfish; Cultural keystone species; Eel; Indigenous ecological knowledge; Indigenous water rights; Lamprey; Salmon; Social-ecological resilience. |
Ano: 2016 |
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Fuhrmann, Marine; Petton, Bruno; Quillien, Virgile; Faury, Nicole; Morga, Benjamin; Pernet, Fabrice. |
Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the... |
Tipo: Text |
Palavras-chave: Bivalve; Herpesviridae; Infection; Disease transmission; Risk analysis. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00350/46100/45781.pdf |
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Rivera-ingraham, Georgina A.; Rocchetta, Iara; Bickmeyer, Ulf; Meyer, Stefanie; Abele, Doris. |
Background: Reactive oxygen (ROS) and nitrogen (RNS) species are produced during normal unstressed metabolic activity in aerobic tissues. Most analytical work uses tissue homogenates, and lacks spatial information on the tissue specific sites of actual ROS formation. Live-imaging techniques (LIT) utilize target-specific fluorescent dyes to visualize biochemical processes at cellular level. Results: Together with oxidative stress measurements, here we report application of LIT to bivalve gills for ex-vivo analysis of gill physiology and mapping of ROS and RNS formation in the living tissue. Our results indicate that a) mitochondria located in the basal parts of the epithelial cells close to the blood vessels are hyperpolarized with high Delta Psi m, whereas... |
Tipo: Text |
Palavras-chave: Bivalve; Gill; Live-imaging; Fluorescence; Mitochondria; ROS; RNS. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00626/73808/74412.pdf |
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Pernet, Fabrice; Lupo, Coralie; Bacher, Cedric; Whittington, Richard J.. |
Emerging diseases pose a recurrent threat to bivalve aquaculture. Recently, massive mortality events in the Pacific oyster Crassostrea gigas associated with the detection of a microvariant of the ostreid herpesvirus 1 (OsHV-1 µVar) have been reported in Europe, Australia and New Zealand. Although the spread of disease is often viewed as a governance failure, we suggest that the development of protective measures for bivalve farming is presently held back by the lack of key scientific knowledge. In this paper, we explore the case for an integrated approach to study the management of bivalve disease, using OsHV-1 as a case study. Reconsidering the key issues by incorporating multidisciplinary science could provide a holistic under-standing of OsHV-1 and... |
Tipo: Text |
Palavras-chave: Bivalve; Disease management; Epidemiology; Multidisciplinary; Risk. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00314/42537/41912.pdf |
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Kermorvant, Claire; Caill-milly, Nathalie; D'Amico, Frank; Bru, Noelle; Sanchez, Florence; Lissardy, Muriel; Brown, Jennifer. |
Bivalves are important components of benthic marine and freshwater ecosystems throughout the world. One of the most exploited bivalves used for human consumption is manila clam (Venerupis philippinarum). In Arcachon Bay (SW France), commercial fishers and scientists have developed a monitoring survey to estimate clam stocks to assist in implementing a sustainable management strategy. The survey design that is currently used is based on standard stratified random sampling (StRS). The survey has been undertaken every 2 years since 2006. Each survey costs approximately (sic) 50 000, with funding provided by similar to 20% of the commercial fishers. The survey is quite expensive, given that this resource is managed mostly at a regional level. In 2016 for... |
Tipo: Text |
Palavras-chave: Venerupis philippinarum; Bivalve; Simulation; Survey; Arcachon Bay; GRTS. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00410/52102/52808.pdf |
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Arzul, Isabelle; Corbeil, Serge; Morga, Benjamin; Renault, Tristan. |
Although a wide range of viruses have been reported in marine molluscs, most of these reports rely on ultrastructural examination and few of these viruses have been fully characterized. The lack of marine mollusc cell lines restricts virus isolation capacities and subsequent characterization works. Our current knowledge is mostly restricted to viruses affecting farmed species such as oysters Crassostrea gigas, abalone Haliotis diversicolor supertexta or the scallop Chlamys farreri. Molecular approaches which are needed to identify virus affiliation have been carried out for a small number of viruses, most of them belonging to the Herpesviridae and birnaviridae families. These last years, the use of New Generation Sequencing approach has allowed increasing... |
Tipo: Text |
Palavras-chave: Molluscs; Viruses; Diseases; Herpesviridae; Bivalve; Abalone. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00371/48206/48319.pdf |
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Botana, Luis M.; Hess, Philipp; Munday, Rex; Nathalie, Arnich; Degrasse, Stacey L.; Feeley, Mark; Suzuki, Toshiyuki; Van Den Berg, Martin; Fattori, Vittorio; Gamarro, Esther Garrido; Tritscher, Angelika; Nakagawa, Rei; Karunasagar, Iddya. |
Background Seafood toxins pose an important risk to human health, and maximum levels were imposed by regulatory authorities throughout the world. Several toxin groups are known, each one with many analogues of the major toxin. Regulatory limits are set to ensure that commercially available seafood is not contaminated with unsafe levels. Scope and Approach The mouse bioassay was used to measure the toxicity in seafood extracts to determine if a sample exceeded regulatory limits. The advantage of this approach was to provide an estimation of the total toxicity in the sample. As instrumental methods of analysis advance and serve as replacements to the mouse bioassay, the challenge is translating individual toxin concentrations into toxicity to determine... |
Tipo: Text |
Palavras-chave: Marine toxins; Toxicity Equivalency Factors; FAO; WHO; Bivalve; Mollusc. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00358/46960/46874.pdf |
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Cavelier, Patricia; Cau, Julien; Morin, Nathalie; Delsert, Claude. |
While our knowledge of bivalve gametogenesis has progressed in recent times, more molecular markers are needed in order to develop tissue imaging. Here, we identified stem cell and mitotic markers to further characterize oyster early gametogenesis, mainly through immunofluorescence microscopy. Intense alkaline phosphatase activity, a non-specific marker for stem cells, was detected on the outer edge of the gonad ducts at the post-spawning stage, suggesting an abundance of undifferentiated cells very early during the sexual cycle. This observation was confirmed using an antibody against Sox2, a transcription factor specific for stem or germline cells, which labeled cells in the gonad duct inner mass and ciliated epithelium early during the initial oyster... |
Tipo: Text |
Palavras-chave: Germline cells; Cell cycle; Reproduction; Bivalve; Marine invertebrates. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00409/52100/52809.pdf |
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Registros recuperados: 74 | |
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